The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. , USA). The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. The drawing shows a standard microplate of 15 mm height covered with a lid. Learn how you can utilize microplate readers to drive landmark discoveries. 0 2. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. TNF standard curve. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 0 out of 5 $ 2,499 $ 1,999. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. • Tel. Microplate Readers Include the M3, M4, M5, and M5. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Table of Contents. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The SpectraMax M5e Multi. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The dual monochromator optics allow. 305 reference level saturation. TR-FRET assay principle. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Microplate Reader Shelf. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. Choose from a. Figure 1. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. . edu) to be added as a trained user in iLab. The cuvette must be in place when you read the plate. Fluorescence Microplate Reader Comparison Chart. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. used. 2. es •. For readers without a cuvette port, these options are not shown in the software. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 00 USD. Choose cables that meet the requirement described in this appendix. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The spectrophotometric. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. spr. M3-M5e_manual. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. The SoftMax® Touch. RAW 264. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. These robust readers have been placed in labs from Antarctica to the. SpectraMax M Series multi-mode microplates are configurable readers with triple. The dual monochromator optics allow the widest. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Wide range of applications The VersaMax reader covers. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Nontreated cells in DMEM medium were used as a control. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Wide range of applications. SpectraMax® M5. Recertification is recommended every two years. The cells were incubated with compounds at the indicated doses for 1 h. Sale price $18,499. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. TR-FRET assay. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. Shop now. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 00 USD. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. In . book Page 7 Wednesday, July 7, 2010 2:09 PM. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. 7°C and the bottom to 36. Microplate Readers Biotek Epoch Microplate. 1 second. Microbiota DNA was. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. FlexStation™ II. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. Label-free detection. 6 for further experiment, so I'm confused which i would choose for OD value determination. 5 12. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. free tracer decreases, resulting in. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. Example: [Drawer]. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. Incubation. For the Live/Dead assay, they were treated. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. 1. We would like to show you a description here but the site won’t allow us. 1. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax® M5. Depending on which. Sale price $16,495. Ltd. Temperature control range from Ambient +4º to 50ºC. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Offer Ends In: 44 Days 10: 22: 56. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Table 1. 40266 Email: drugdiscoverytech@lifetech. ® reader to the computer. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. 0 out of 5 $ 24,695. Figure 3. The guide also covers the software features, the specifications, and the accessories of the systems. SpectraMax® ABS Plus. (Shanghai, China). We would like to show you a description here but the site won’t allow us. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. Microplate reader settings. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. ) by nonlinear regression using. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. 7 cells were co-transfected with 0. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Five. Lower limits of detection for ATP determined using the ATPlite . keeping with best practices, reader performance should. 6 0. Examples: “Press carefully to engage,” “ Do not. 5°C at 37°C. 2. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. flexibility of monochromator-based systems. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. Page 28 Chapter 5: Read Modes and Read Types 1. Microplate Readers Biotek Synergy H1 Hybrid Microplate. 0 out of 5 $ 17,999 $ 16,995. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. SpectraMax® iD5. 40266 Email: drugdiscoverytech@lifetech. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. 67. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. 04 to 3. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Supersedes 1. com A. Add to Cart. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. ATP standard curves read on SpectraMax microplate readers. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Figure 1. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax® M5e. free tracer decreases, resulting in an. The plate reader can measure multiple readouts, including UV and visible absorbance. SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. The assay applies fluorescence polarization to quantify Fc-containing IgG. edu) to be added as a trained user in iLab. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. It cannot be used for luminescence measurements. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Bookings are free, but you will need to use iLab to book. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. When the user applies a set temperature of 37°C, the top is heated to 37. SpectraMax® ABS. Wash Buffer Concentrate was diluted 10-fold. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. Protocol Name Version 6. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The acceptance. . SpectraMax® Aquamax. 58% for the detection module and 2. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Amino acids assay. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. When a feature or capability applies to only certain readers, this exception is noted. 54 1. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. This reader's dual monochromators for. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. A: Microplate Preparation. Learn how you can utilize microplate readers to drive landmark discoveries. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Step 2. book Page 7 Wednesday, July 7, 2010 2:09 PM. SpectraMax® M4. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax® ABS Plus. Fill a clean plate with water. analysis, controls the SpectraMax Multi-Mode Microplate Readers. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Bioz Stars score: 86/100, based on 1 PubMed citations. At the end of this period, cells were exposed to FA (1 mM) for 1 h. Italic and boldface type are used for emphasis. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Wide range of applications. The assay window spanned four orders of magnitude. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). We would like to show you a description here but the site won’t allow us. 4 Max StDev 1. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 6-384 (96- and 384-well with pipetting) 6-384. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. However, when a specific probe is not available, or it causes off-target effects on the. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. A: Microplate Preparation. p. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. 2 only) SpectraMax M-series Microplate readers. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. 312 gain calibration failed. 5. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. both cuvette-based and microplate reader applications on the same instrument. Plate Shaking. Introduction. Five preferred detection modes. The PBS solution (10. The spectrophotometric. Molecular Devices Corporation. Note: In this user guide, SpectraMax ® refers to several SpectraMax . The advantage of using labeled probes is detection specificity. IMAP assays are based on binding of. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. 2. Self Service Available. Instrument settings are shown in Figure 3. You should get comparable results from top and bottom reads using EC50's to compare reads. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. "With this complete portfolio of multi-mode configurations, we. AquaMax® 4000. 1. When a feature or capability applies to only certain readers, this exception is noted. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Five preferred detection modes. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). SpectraMax M5 reader settings are shown. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. HTRF-compatible SpectraMax Multi-Mode Readers. Spectramax M5 microplate reader: Molecular Devices: N/A:. . No filter change with dual monochromator optics. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Cables. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Temperature variation ± 0. We use Spectramax M5 microplate reader in our lab. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 2 only). This. 5 87. SpectraMax M5 Multi-Mode Microplate Reader. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). 0 . 1. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. 2. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. es •. Example: [Drawer]. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. The Multiskan Sky microplate reader is an absorbance plate reader. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 7. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 4. Most papers they cultured bacteria to OD600>0. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. 2. Category 2 and 3 microplate readers use gradient heating as well. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. com. l. Compact and tunable absorbance microplate readers DATA SHEET. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Key applications: ELISA, nucleic acid, endotoxin testing. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. AquaMax® 2000. flexibility of monochromator-based systems. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Turn on the microplate reader. 49 2. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. 1–0. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. View the Molecular Devices website. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Shop now. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Molecular Devices Spectramax M5 Microplate Reader. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. 63 1. 4. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Read More. When a. All associated StakMax® stackers. The CellTiter-Glo assay from Promega uses luciferase. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). D. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. Make sure the microplate is flat against. 6-384. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Molecular Devices Spectramax M5 Microplate Reader. Figure 4: Transcreener ADP. Sensitive fluorescent quantitation of DNA with the Quant-iT. Page 28 Chapter 5: Read Modes and Read Types 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. Go to Product Details. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. 306 plate air cal. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers.